Fig. 3
From: DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice
DNMT1 knockdown promoted PPARα expression in HG-treated HRCPs. HRCPs were treated with 5 mM glucose, 30 mM glucose or 5 mM glucose combined with 25 mM mannose. A WB was performed to assess the expression of DNMT1, DNMT3A and DNMT3B in HRCPs. B The interaction between DNMT1 and PPARα promoter was examined by ChIP assay. HRCPs were transfected with si-DNMT1 or siRNA, and then treated with 30 mM glucose. QRT-PCR (C) and WB (D) were performed to assess PPARα gene and protein expression in HRCPs. **P < 0.01 vs. NG; &P < 0.05 vs. HG + siRNA