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Fig. 1 | Biological Research

Fig. 1

From: DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice

Fig. 1

The methylation levels of PPARα in HRCPs were enhanced by HG treatment. HRCPs were treated with 5 mM glucose, 30 mM glucose or 5 mM glucose combined with 25 mM mannose. A The methylation levels of PPARα in HRCPs were detected by MSP. QRT-PCR (B) and WB (C) were performed to assess PPARα gene and protein expression in HRCPs. HRCPs were treated with 30 mM glucose combined with 10 mM DAC. QRT-PCR (D) and WB (E) were performed to examine the gene and protein expression of PPARα in HRCPs. L marker, M methylation, U unmethylation. **P < 0.01 vs. NG; #P < 0.05 vs. HG

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