Fig. 4
From: Cisplatin-induced hydroxyl radicals mediate pro-survival autophagy in human lung cancer H460 cells
Elevated hydroxyl radicals in cisplatin-treated human lung cancer cells. a Augmented level of cellular ROS and hydroxyl radicals (OH·) in lung cancer H460 cells after treatment with 50 µM cisplatin for 6 h, as determined by flow cytometric analysis with DCFH2-DA and HPF, respectively, b without alteration of superoxide anions (O2·−) levels (flow cytometry chromatogram of DHE). c Detection with Amplex red clearly demonstrated the increased relative level of cellular hydrogen peroxide (H2O2) in H460 cells after culture with 500 µM H2O2, but not by 50 µM cisplatin. Data are shown as the mean ± SD (n = 3). *p < 0.05 versus untreated control cells