Fig. 5

Synergistic effect between MH and DOX modulated signalling pathways that involved in HepG2 cells’ proliferation. a (Left upper panel); Protein expression levels of Bax, Bcl-2, ERK, pERK1/2, mTOR, S6K, oncogenic β-catenin, and cyclin D1 were detected by Western blot analysis. a (Right upper panel); Relative protein expression levels in HepG2 cells were quantified by Quantity One software. b (Left lower panel); Protein expression level of cleaved PARP was detected by Western blot analysis. b (Right lower panel); Relative protein expression levels in HepG2 cells were quantified by Quantity One software. HepG2 cells were treated with MH, DOX, or combined treatment for 48 h. The cell lysate was subjected to 10% SDS-PAGE. Proteins were transferred to nitrocellulose membrane and probed with the indicated antibodies. Anti-β-actin was used as a loading control. Protein expressions were quantified normalized to β-actin and controls. Numbers represent the fold of change. The experiment was done in triplicate. Data are presented as mean M ± SD. P-value was calculated versus control cells: **P < 0.01 and ***P < 0.001