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Fig. 2 | Biological Research

Fig. 2

From: The role of circular RNA circ_0008285 in gestational diabetes mellitus by regulating the biological functions of trophoblasts

Fig. 2

Validation of circ_0008285 and circ_0001173 in sequencing samples. a The circularity of circ_0008285 and circ_0001173 verified in the HTR-8/SVneo cells by RT-PCR. circ_0008285 and circ_0001173 were amplified by divergent primers in cDNA and not in gDNA. b Sanger sequence analysis was used to confirm the splicing site. c The stability of circ_0008285 and circ_0001173 were evaluated via the RNase R treatment experiment. d The expression levels of the circ_0008285 and circ_0001173 were validated by qRT-PCR. GDM, gestational diabetes mellitus; HC, healthy pregnant controls. **P < 0.01, ****P < 0.0001

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Biological Research

ISSN: 0717-6287

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