Fig. 6

Knockdown of HDAC1 could reverse the effects of anti-miR-135b-5p on cell proliferation, apoptosis and inflammatory of ox-LDL-treated HUVECs. a The expression of HDAC1 was measured in control, ox-LDL, ox-LDL + anti-miR-NC, ox-LDL + anti-miR-135b-5p, ox-LDL + anti-miR-135b-5p + si-NC and ox-LDL + anti-miR-135b-5p + si-HDAC1 groups using western blot, the measurements of the following indicators are grouped in this experiment b MTT assay was applied to measure cell proliferation. c Flow cytometry was used to detect cell apoptosis. d Western blot assay detected the protein expression of Bcl-2, Bax and cleaved-caspase-3. e–h ELISA was used to assess the level of IL-1β, IL-6, TNF-α and IL-8. i Western blot assay detected the protein expression of VCAM1, ICAM1 and E-selectin. ‘ + ’ was added, ‘−’ was blank*. Each experiment was performed in triplicate p < 0.05