Fig. 4

The effects of high circRSF1 expression on cell proliferation, apoptosis and inflammatory of ox-LDL-treated HUVECs was reversed by miR-135b-5p overexpression. a The expression of miR-135b-5p was measured in control, ox-LDL, ox-LDL + vector, ox-LDL + circRSF1, ox-LDL + circRSF1 + miR-NC and ox-LDL + circRSF1 + miR-135b-5p groups using qRT-PCR, the measurements of the following indicators are grouped in this experiment. b MTT assay was applied to measure cell proliferation. c Flow cytometry was used to detect cell apoptosis. d Western blot assay detected the protein expression of Bcl-2, Bax and cleaved-caspase-3. e–h ELISA was used to assess the level of IL-1β, IL-6, TNF-α and IL-8 in control, ox-LDL, ox-LDL + vector, ox-LDL + circRSF1, ox-LDL + circRSF1 + miR-NC and ox-LDL + circRSF1 + miR-135b-5p groups. i Western blot assay detected the protein expression of VCAM1, ICAM1 and E-selectin. ‘ + ’ was added, ‘−’ was blank. Each experiment was performed in triplicate *p < 0.05