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Fig. 2 | Biological Research

Fig. 2

From: CircRNA RSF1 regulated ox-LDL induced vascular endothelial cells proliferation, apoptosis and inflammation through modulating miR-135b-5p/HDAC1 axis in atherosclerosis

Fig. 2

Induction of circRSF1 promoted cell proliferation, and inhibited apoptosis and inflammatory of ox-LDL-treated HUVECs. a The expression of circRSF1 was measured in control, ox-LDL, ox-LDL + vector and ox-LDL + circRSF1 groups using qRT-PCR. b MTT assay was applied to measure cell proliferation in control, ox-LDL, ox-LDL + vector and ox-LDL + circRSF1 groups. c Flow cytomety was used to detect cell apoptosis in control, ox-LDL, ox-LDL + vector and ox-LDL + circRSF1 groups. d Western blot assay detected the protein expression of Bcl-2, Bax and cleaved-caspase-3 in control, ox-LDL, ox-LDL + vector and ox-LDL + circRSF1 groups. eh ELISA was used to assess the level of IL-1β, IL-6, TNF-α and IL-8 in control, ox-LDL, ox-LDL + vector and ox-LDL + circRSF1 groups. i The protein expression of VCAM1, ICAM1 and E-selectin was detected in control, ox-LDL, ox-LDL + vector and ox-LDL + circRSF1 groups. ‘ + ’ was added, ‘−’ was blank *p < 0.05

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