Fig. 5

HMGA1 is the downstream target of the MALAT1-miR-195a-5p axis. a Putative miR-195a-5p binding sites in HMGA1. The potential complementary residues are shown in red. b Relative luciferase activity of wild-type and 3ʹ-UTR mutant constructs of HMGA1 cotransfected with miR-195a-5p mimics and miRNA negative control. ***p < 0.001 versus the miRNA negative control group using one-way ANOVA followed by the Holm-Sidak test. Real-time PCR (c) and western blot (d) analyses of HMGA1 expression in HT22 cells transduced with miR-195a-5p inhibitor. n = 3. **p < 0.01 and ***p < 0.001 versus the control group using the Student t test (e) western blot analyses HMGA1 expression in HT22 cells exposure to OGD/R. **p < 0.01 and ***p < 0.001 versus the control group using the Student t test