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Fig. 3 | Biological Research

Fig. 3

From: Lotus leaf flavonoids induce apoptosis of human lung cancer A549 cells through the ROS/p38 MAPK pathway

Fig. 3

Effect of lotus leaves flavonoids (LLF) on the apoptosis marker Annexin-V over A549 lung cancer cells. A549 cells were exposed to LLF at different concentrations and the fluorescence intensity after Annexin-V stain was measured by flow cytometry. a Control: untreated A549 cells; b A549 cells treated with LLF at 125 µg/mL for 48 h; c A549 cells treated with LLF at 250 µg/mL for 48 h; d A549 cells treated with LLF at 500 µg/mL for 48 h. Frequency histogram represents the median fluorescence intensity (MFI) against the cell count. The increase in the MFI represents an increase in the number of the cells positive for the apoptosis mark Annexin-V (n = 6)

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