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Fig. 3 | Biological Research

Fig. 3

From: Exo70 intracellular redistribution after repeated mild traumatic brain injury

Fig. 3

Exo70 localization is increased in forebrain synapses upon mTBI induction. a Forebrains from two-month-old male mice were obtained and subcellular fractionation was carried out. Homogenized (H), cytosol (CYT), microsome (MIC), and synaptosome (SYN) fractions were obtained. 20 µg of protein samples were resolved in a 10% SDS-PAGE and transferred to PVDF membranes. Membranes were incubated with the respective antibodies shown in the figure. Membranes were stripped and tested again with the indicated antibodies. b Proteins distribution were analyzed with densitometric analysis by comparing signal intensity from each fraction with homogenized. Mean values ± SEM are shown. c Two-month-old male mice were subjected to mTBI and microsome and synaptosome fractions were obtained. 30 µg of protein samples were analyzed. PDI/Tubulin was used as loading controls. d The graph shows the Exo70 densitometric analysis normalized with loading controls. Values represent means ± SEM, n = 3 mice per experimental group. Statistical differences were determined by an unpaired t-test comparing Sham and mTBI. **p < 0.01

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