Fig. 7

Testosterone increases expression levels of glycolytic enzymes in cardiac hypertrophy in vivo. The rats were ORX and treated with vehicle (ORX) or Testosterone (ORX + T). Control group were performed with normal rats. mRNA levels were assessed using RT-qPCR for a β-mhc; b Hk2; and c Pfk2 (n = 4 rats per each experimental condition). These genes were normalized relative to 18S mRNA expression, and the values shown here correspond to target-gene/18S mRNA ratios. Data are presented as means ± SEM; P-values were determined using ANOVA followed by Tukey’s post hoc test; #P < 0.05 vs. ORX group