Fig. 6

AMPK is required in testosterone-induced cardiomyocyte hypertrophy. Cardiomyocytes were pretreated with 2 µM CC and stimulated with 100 nM testosterone by 24 h. a Cardiomyocyte cellular area. b β-mhc mRNA levels were evaluated in cells treated with 100 nM testosterone for 10 h in the presence or absence of CC (2 µM). The mRNA levels were normalized to 18S mRNA expression. Data are expressed as mean ± SEM of at least three independent experiments. *P < 0.05, **P < 0.01 vs. control; ##P < 0.01, ###P < 0.001 vs. testosterone