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Fig. 3 | Biological Research

Fig. 3

From: Testosterone activates glucose metabolism through AMPK and androgen signaling in cardiomyocyte hypertrophy

Fig. 3

AR is required for glucose metabolic changes in testosterone-induced cardiomyocyte hypertrophy. a ECAR kinetics in cells treated with bicalutamide (2 µM) and testosterone 100 nM for 24 h. b The increase in glycolysis and maximal glycolytic capacity induced by 100 nM testosterone were blocked by bicalutamide. c Cardiomyocytes were stimulated with 100 nM testosterone for 24 h and were then incubated with 2-NBDG for 20 min, intracellular fluorescence was measured in the presence or absence of bicalutamide (2 µM). AR inhibition abolished testosterone-stimulated glucose uptake. d Cardiomyocytes were pretreated for 30 min with bicalutamide (2 µM). Levels of mRNA for Hk2 were evaluated in cells treated with testosterone 100 nM for 10 h in the presence or absence of bicalutamide (2 µM). Data are expressed as mean ± SEM of at least three independent experiments. P-values were determined using ANOVA followed by Tukey’s post hoc test; *P < 0.05, ***P < 0.001 vs. control; #P < 0.05, ###P < 0.001 vs. testosterone treated cells

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