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Table 4 A list of differentially expressed proteins in the hippocampus with fundamental roles in the regulation of cell death

From: Proteome profiling of different rat brain regions reveals the modulatory effect of prolonged maternal separation on proteins involved in cell death-related processes

Protein ID Gene name Protein name Y S/C M S/C F S/C C M/F S M/F Protein function
Pdk2 [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial ↓ (0.46) p53 activation decreases Pdk2 transcription [92]
P97570 Pla2g6 85/88 kDa calcium independent phospholipase A2 S C M tBID and Bax augments Pla2g6 (iPLA2) activity via ROS production leading to changes in the MOM [93]
Overexpression of iPLA2 increased the rate of apoptosis, iPLA2 is cleaved by caspase-3 [94]
β-cell apoptosis is attributable to the modulation of 5′SS selection in Bcl-X pre-mRNA by bioactive lipids modulated by iPLA2 [95]
Apc Adenomatosis polyposis coli C F APC can regulate apoptosis by governing the level of caspase-3 [133]
P23928 Cryab Alpha-crystallin B chain C Cryab prevents apoptosis; it binds to caspase-3 [134], Bax and Bcl-XS [135] and p53 [136]
Cryab gene is a targer of p53 and p53-dependent apoptosis is affected by Cryab expression [137]
Bax Apoptosis regulator BAX S C F M Pro-apoptotic member of Bcl-2 family, its activation permeabilizes the MOM during apoptosis [96]
Deletion of Bax is sufficient to inhibit apoptosis [97]
Bax is cleaved by active calpains [80]
E9PST5 Acin1 Apoptotic chromatin condensation inducer 1 ↑ (2.02) ↓ (0.36) ↑ (3.30) Acin1 can induce apoptotic chromatin condensation after its cleavage by caspase-3 [137]
Q91XJ1 Becn1 Beclin-1 N C C N Beclin-1-induced autophagy is inversely correlated with apoptosis [13]
Q6IN33 Rcan1 Calcipressin-1 C S M F Calcineurin regulation [138]; Rcan1 absence enhances calcineurin activity and Fas ligand expression [98]
Rcan2 Calcipressin-2 S N S N F Calcineurin regulation [138]
Crp C-reactive protein C Crp can induce the upregulation of p53 and increase caspase-3 activity [139]
Ephb2 Eph receptor B2 S C F M Ephb2 can prevent against Fas-triggered apotosis by inhibition of caspase-8 and caspase-3 [140]
Q8R2E7 Fadd FAS-associated death domain protein S C F Pro-apoptotic adapter protein involved in extrinsic pathway of apoptosis [100]
Overexpression of FADD promotes apoptosis [103]
Grid2 Glutamate ionotropic receptor, delta-2 N N C F Gain-to-function mutation of Grid2 induces neuronal death [105]; this mutation is associated with increased expression of Bax, Bcl-XS, caspase-3 and -8 [106]
TNF-α has a role in regulation of Grid2 gene expression, which can be a suppressor in TNF-induced neurodegeneration [107]
Hspb1 Heat shock 27 kDa ↑ (2.14) ↑ (2.39) Hspb1 indirectly suppresses stress-induced Bax oligomerization and translocation to mitochondria; it can inhibit caspase-3 activity [141]
Hspb1 can mediate Bad inactivation [142]
Q8VH49 Higd1a HIG1 domain family 1A, mitochondrial C N C F N Higd1a is a survival factor and is associated with caspase-3 activation [109, 110]
P31423 Grm4 Metabotropic glutamate receptor 4 S S N N M Decreased expression of GRM4 gene is associated with apoptosis of cerebellar granule neurons [86]
Activation of Grm4 can activate pro-caspase 8/9/3 and disrupt the balance of Bcl-2/Bax expression [113]
P2rx7 P2X purinoceptor 7 S P2rx7s mediate caspase-8 and caspase-3 dependent apoptosis [115]
Its activation is associated with Ca2+ responses and TNF-α production [116,117,118]
P27008 Parp1 Poly [ADP-ribose] polymerase 1 C F Parp1 is cleaved by caspase-3 [21]
Mpv17 Protein Mpv17 N F F The increase of Mpv17 expression can be accompanied by the enhanced expression of p53, Bax, cyt c and active caspase-3 and decreased expression of Bcl-2 in the pathological proces [119]
P70600 Ptk2b Protein-tyrosine kinase 2-beta ↓ (0.49) Overexpression of Ptk2b can induce apoptosis [143]
B1WC67 Slc25a24 RCG29001 S C M A survival factor, knock-down of Slc25a24 led to reduction of Ca2+ buffering capacity and sensitized cellts to cell death induced by mitochondrial permeability transition [120]
Q63639 Aldh1a2 Retinal dehydrogenase 2 N S S N Aldh1a2 mediates conversion of retinol into active retinoic acid, which the expression of Bcl-2, Bax, Bid, caspase-8 and -3 [122]
D4ADQ1 Rrm2b Ribonucleotide reductase M2 B (TP53 inducible) N C F N Loss of Rrm2b can increase apoptosis [123]
Rrm2b expression is induced by stress [124]
Rrm2b cleavage is mediated by caspase-8 and -3 [125]
Rbm10 RNA-binding protein 10 N F F Rbm10 knock-down can decrease caspase activation [126]
Rbm10 regulates alternative splicing of Fas and Bcl-X genes [127]
Rbm10 expression is associated with increased apoptosis [128]
Stat5b;Stat5a Signal transducer and activator of transcription S M Stat5 regulate expression of Bcl-2 and Bcl-XL and the level of caspase-3 [129,130,131]
Tp53bp2 Tumor protein p53-binding protein 2 S N N M Tp53bp2 binds to p53 and Bcl-2; it can alter p53 protein conformation and enhance the binding activity of p53 to the promotes of Bax [132]
  1. C protein detected only in samples of the cerebral cortex from control rats, S protein detected only in samples of the cerebral cortex from maternally separated rats, M protein detected only in samples of the cerebral cortex from adult male rats, F protein detected only in samples of the cerebral cortex from adult female rats, N protein not detected in the samples, –, unchanged protein expression between the samples; ↓, protein down-regulated in samples of the cerebral cortex from maternally separated rats; ↑, protein up-regulated in samples of the cerebral cortex from maternally separated