Fig. 1

Expressions of YAP1 and JAG1 in breast cancer cells and tissues were upregulated. a RT-PCR and its quantification were used to determine the mRNA levels of linc-OIP5, YAP1 and JAG1, which were differentially expressed in normal and cancerous breast cells. b Immunohistochemistry analysis of breast cancer tissue samples confirmed that YAP1 and JAG1 are significantly higher in grade III than grade I and YAP1 in cancer tissues also displayed robust nuclear localization. c Immunoblot analysis and its quantification were performed to check the expression levels of YAP1 and JAG1 in MDA-MB-231 and MCF-7 cells. Fold changes were obtained by normalizing against β-actin. Graphs are means of indicated stained area in 5 samples and error bars indicate s.d. Magnification×200. *P < 0.05, ** P < 0.01, ***P < 0.001