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Fig. 3 | Biological Research

Fig. 3

From: Knockdown of lncRNA TUG1 inhibits hippocampal neuronal apoptosis and participates in aerobic exercise-alleviated vascular cognitive impairment

Fig. 3

TUG1 knockdown reduces neuronal apoptosis by upregulating BDNF. a RIP results showed that TUG1 was enriched in the anti-BDNF immunoprecipitants. *P < 0.05 vs. the IgG group. b BDNF was enriched in the TUG1-pulled down compounds. HT22 cells were divided into four groups, the OGD group (cells were reoxygenated for 24 h after OGD treatment for 1 h), the OGD + LV-shRNA group (cells were transfected with LV-shRNA for 72 h before the OGD treatment), the OGD + LV-sh-TUG1 group (cells were transfected with LV-sh-TUG1 for 72 h before the OGD treatment), and the OGD + LV-sh-BDNF group (cells were transfected with LV-sh-BDNF for 72 h before the OGD treatment). c The TUG1 level was detected by qRT-PCR. d The BDNF mRNA level was detected by qRT-PCR. e, f The protein expressions of BDNF, cleaved-Caspase 3, and Bcl-2 were detected by western blotting. g Cell apoptosis was detected by flow cytometry. The experiments were repeated three times (n = 3). *P < 0.05 vs. the OGD + LV-shRNA group; **P < 0.05 vs. the OGD + LV-sh-TUG1 group

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