Table 1 The sequence of gRNAs and primers
From: Caspase-7 deficiency in Chinese hamster ovary cells reduces cell proliferation and viability
Oligoes | Caspase 7 target site | sequences |
|---|---|---|
Exon 4 | agatggcgtgacgccaataa | Fw: CACCgAGATGGCGTGACGCCAATAA Rev: AAACTTATTGGCGTCACGCCATCT |
Exon 4 + PAM | agatggcgtgacgccaataaagg | Fw: caccgCCTTTATTGGCGTCACGCCATCT Rev: aaacAGATGGCGTGACGCCAATAAAGGc |
Exon 5 | gatacgctttaggcatgccg | Fw: CACCGATACGCTTTAGGCATGCCG Rev: AAACCGGCATGCCTAAAGCGTATC |
Exon 5 + PAM | gatacgctttaggcatgccgagg | Fw: caccCCTCGGCATGCCTAAAGCGTATC Rev:aaacGATACGCTTTAGGCATGCCGAGG |
PX-460-1 primers | – | Fwd: GCCTTTTGCTGGCCTTTTGCTC Rev:CGGGCCATTTACCGTAAGTTATGTAACG |
Genomic primers | – | Fwd: CTGAGGAGGACCACAGCAACTC Rev: AACTGTGGAGTAAGCGAAGAGAA |