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Table 4 Protein expression of selected cell cycle proteins and elastin in lung homogenates measured by enzyme immunoassay (EIA) in mice at 4 and 12 weeks

From: Short-term perinatal oxygen exposure may impair lung development in adult mice

Protein 4 Weeks 12 Weeks
21% O2 40% O2 100% O2 21% O2 40% O2 100% O2
CCNB1 27.32 ± 3.54 34.60 ± 6.29* 29.08 ± 3.26 28.29 ± 3.54 21.59 ± 4.73 25.04 ± 4.65
MKI67 17.05 ± 2.98 25.16 ± 1.4* 19.24 ± 2.38 26.63 ± 5.67 22.35 ± 4.15 25.51 ± 7.39
CDKN1A (p21) 12.60 ± 0.31 12.58 ± 0.28 12.25 ± 0.28 14.33 ± 3.68 12.89 ± 0.30 12.71 ± 0.08
Trp63 2.46 ± 2.71 3.55 ± 2.51 0.66 ± 0.15 1.67 ± 0.13 1.48 ± 1.01 1.37 ± 0.66
ELN 1.14 ± 0.37 0.96 ± 0.22 0.48 ± 0.24* 0.23 ± 0.20 0.57 ± 0.40 0.62 ± 0.42
  1. The mice were exposed to 30 min of oxygen exposure (21%O2, 40%O2, or 100%O2) within six hours after birth
  2. Values were expressed as mean ± SD (N = 6 in each group, each time point)
  3. CCNB1 cyclin B1, Ki67 marker of proliferation Ki-67, CDKN1A (p21) cyclin-dependent kinase inhibitor 1A, Trp63 tumor protein 63, ELN elastin, Cell cycle proteins and elastin expressed as pg/µg lung protein
  4. *p < 0.05 vs. 21%O2 group
  5. p < 0.05 vs. 100% O2 group (Fisher's Post-Hoc test, ANOVA)
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