Skip to main content

Table 4 Protein expression of selected cell cycle proteins and elastin in lung homogenates measured by enzyme immunoassay (EIA) in mice at 4 and 12 weeks

From: Short-term perinatal oxygen exposure may impair lung development in adult mice

Protein

4 Weeks

12 Weeks

21% O2

40% O2

100% O2

21% O2

40% O2

100% O2

CCNB1

27.32 ± 3.54

34.60 ± 6.29*

29.08 ± 3.26

28.29 ± 3.54

21.59 ± 4.73

25.04 ± 4.65

MKI67

17.05 ± 2.98

25.16 ± 1.4*†

19.24 ± 2.38

26.63 ± 5.67

22.35 ± 4.15

25.51 ± 7.39

CDKN1A (p21)

12.60 ± 0.31

12.58 ± 0.28

12.25 ± 0.28

14.33 ± 3.68

12.89 ± 0.30

12.71 ± 0.08

Trp63

2.46 ± 2.71

3.55 ± 2.51

0.66 ± 0.15

1.67 ± 0.13

1.48 ± 1.01

1.37 ± 0.66

ELN

1.14 ± 0.37

0.96 ± 0.22

0.48 ± 0.24*

0.23 ± 0.20

0.57 ± 0.40

0.62 ± 0.42

  1. The mice were exposed to 30 min of oxygen exposure (21%O2, 40%O2, or 100%O2) within six hours after birth
  2. Values were expressed as mean ± SD (N = 6 in each group, each time point)
  3. CCNB1 cyclin B1, Ki67 marker of proliferation Ki-67, CDKN1A (p21) cyclin-dependent kinase inhibitor 1A, Trp63 tumor protein 63, ELN elastin, Cell cycle proteins and elastin expressed as pg/µg lung protein
  4. *p < 0.05 vs. 21%O2 group
  5. †p < 0.05 vs. 100% O2 group (Fisher's Post-Hoc test, ANOVA)