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Table 2 Cell cycle gene expression by real-time PCR array in lung homogenate of mice at 4- and 12-weeks of age exposed to 30 min of 100%O2 within 6 h of birth

From: Short-term perinatal oxygen exposure may impair lung development in adult mice

Gene symbol

Gene description

Fold change in gene expression

100%O2 Group—4 weeks (Control Group—21%O2 4 weeks)

Over-expressed genes

 Cdk4

Cyclin-dependent kinase 4

4.07 (0.01–15.8)

 Dst

Dystonin

3.45 (0.001–12.3)

 Mre11a

Meiotic recombination 11 homolog A

2.06 (0.001–4.38)

 Mdm2

mouse 3T3 cell double minute 2

2.01 (0.54–3.49)

 Msh2

MutS homolog 2 (E. coli)

2.91 (0.01–10.32)

 Npm2

Nucleoplasmin 2

2.31 (0.02–4.62)

 Psmg2

Proteasome assembly chaperone 2

3.28 (0.01–10.3)

 Rbl1

Retinoblastoma-like protein 1

2.70 (0.01–7.56)

 Shc1

Src homology 2 transforming protein C1

2.52 (0.01–5.85)

 Slfn1

Schlafen 1

2.01 (0.51–3.45)

Under-expressed genes

 Ak1

Adenylate kinase 1

0.34 (0.01–0.68)

 Abl1

Non-receptor tyrosine protein kinase

0.31 (0.01–0.98)

 Ccna2

Cyclin A2

0.35 (0.14–0.57)*

 Ccnb1

Cyclin B1

0.08 (0.001–0.31)

 Ccnb2

Cyclin B2

0.38 (0.13–0.64)*

 Cdkn1a

Cyclin-dependent kinase inhibitor 1A

0.50 (0.31–0.69)*

 Gpr132

G protein-coupled receptor 132

0.32 (0.001–1.61)

 Mcm3

Minichromosome maintenance complex-3

0.17 (0.001–0.71)

 Mki67

Antigen identified by monoclonal antibody Ki-67

0.39 (0.21–0.58)*

 Ppm1d

Protein Phosphatase 1D

0.28 (0.001–0.97)

 Ran

RAs-related nuclear protein

0.13 (0.01–0.62)

 Rbl2

Retinoblastoma-like protein-2

0.22 (0.01–0.81)

 Sfn

Stratifin

0.38 (0.001–0.94)

100%O2 Group—12 Weeks (Control Group—21%O2 12 weeks)

Over-expressed genes

 Atm

Ataxia Telangiectasia Mutated

2.4 (0.01–7.38)

 Ccnb1

Cyclin B1

2.55 (0.001–6.75)

 Ccnb2

Cyclin B2

3.38 (0.001–11.3)

 Ccnc

Cyclin C

2.28 (0.01–7.15)

 Trp63

Transformation related protein p63

2.93 (0.01–8.79)

Under-expressed genes

 Abl1

Non-receptor tyrosine protein kinase

0.33 (0.01–0.88)

 Inha

Inhibin alpha

0.52 (0.32–0.73)*

 Skp2

S-phase kinase-associated protein 2 (p45)

0.62 (0.46–0.79)*

  1. Genes were considered over-expressed (Fold Change ≥ 2.0) or under-expressed (Fold Change ≤ 0.5) relative to the control group (21%O2). The fold change (FC) in gene expression in 21%O2 group = 1.0; values expressed as FC with 95% confidence intervals
  2. Housekeeping genes: Gapdh (Glyceraldehyde-3-phosphate dehydrogenase), Hprt (Hypoxanthine guanine phosphoribosyl transferase) & Actb (Actin, beta); N = six mice / group, each time-point
  3. *p < 0.01 vs control group
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Biological Research

ISSN: 0717-6287

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