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Table 2 Cell cycle gene expression by real-time PCR array in lung homogenate of mice at 4- and 12-weeks of age exposed to 30 min of 100%O2 within 6 h of birth

From: Short-term perinatal oxygen exposure may impair lung development in adult mice

Gene symbol Gene description Fold change in gene expression
100%O2 Group—4 weeks (Control Group—21%O2 4 weeks)
Over-expressed genes
 Cdk4 Cyclin-dependent kinase 4 4.07 (0.01–15.8)
 Dst Dystonin 3.45 (0.001–12.3)
 Mre11a Meiotic recombination 11 homolog A 2.06 (0.001–4.38)
 Mdm2 mouse 3T3 cell double minute 2 2.01 (0.54–3.49)
 Msh2 MutS homolog 2 (E. coli) 2.91 (0.01–10.32)
 Npm2 Nucleoplasmin 2 2.31 (0.02–4.62)
 Psmg2 Proteasome assembly chaperone 2 3.28 (0.01–10.3)
 Rbl1 Retinoblastoma-like protein 1 2.70 (0.01–7.56)
 Shc1 Src homology 2 transforming protein C1 2.52 (0.01–5.85)
 Slfn1 Schlafen 1 2.01 (0.51–3.45)
Under-expressed genes
 Ak1 Adenylate kinase 1 0.34 (0.01–0.68)
 Abl1 Non-receptor tyrosine protein kinase 0.31 (0.01–0.98)
 Ccna2 Cyclin A2 0.35 (0.14–0.57)*
 Ccnb1 Cyclin B1 0.08 (0.001–0.31)
 Ccnb2 Cyclin B2 0.38 (0.13–0.64)*
 Cdkn1a Cyclin-dependent kinase inhibitor 1A 0.50 (0.31–0.69)*
 Gpr132 G protein-coupled receptor 132 0.32 (0.001–1.61)
 Mcm3 Minichromosome maintenance complex-3 0.17 (0.001–0.71)
 Mki67 Antigen identified by monoclonal antibody Ki-67 0.39 (0.21–0.58)*
 Ppm1d Protein Phosphatase 1D 0.28 (0.001–0.97)
 Ran RAs-related nuclear protein 0.13 (0.01–0.62)
 Rbl2 Retinoblastoma-like protein-2 0.22 (0.01–0.81)
 Sfn Stratifin 0.38 (0.001–0.94)
100%O2 Group—12 Weeks (Control Group—21%O2 12 weeks)
Over-expressed genes
 Atm Ataxia Telangiectasia Mutated 2.4 (0.01–7.38)
 Ccnb1 Cyclin B1 2.55 (0.001–6.75)
 Ccnb2 Cyclin B2 3.38 (0.001–11.3)
 Ccnc Cyclin C 2.28 (0.01–7.15)
 Trp63 Transformation related protein p63 2.93 (0.01–8.79)
Under-expressed genes
 Abl1 Non-receptor tyrosine protein kinase 0.33 (0.01–0.88)
 Inha Inhibin alpha 0.52 (0.32–0.73)*
 Skp2 S-phase kinase-associated protein 2 (p45) 0.62 (0.46–0.79)*
  1. Genes were considered over-expressed (Fold Change ≥ 2.0) or under-expressed (Fold Change ≤ 0.5) relative to the control group (21%O2). The fold change (FC) in gene expression in 21%O2 group = 1.0; values expressed as FC with 95% confidence intervals
  2. Housekeeping genes: Gapdh (Glyceraldehyde-3-phosphate dehydrogenase), Hprt (Hypoxanthine guanine phosphoribosyl transferase) & Actb (Actin, beta); N = six mice / group, each time-point
  3. *p < 0.01 vs control group
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