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Table 1 Cell cycle gene expression by real-time PCR array in lung homogenate of mice at 4- and 12-weeks of age exposed to 30 min of 40%O2 within 6 h of birth

From: Short-term perinatal oxygen exposure may impair lung development in adult mice

Gene symbol Gene description Fold change in gene expression
40%O2 Group—4 weeks (Control Group—21%O2 4 weeks)
Over-expressed genes
 Cdk4 Cyclin-dependent kinase 4 4.26 (0.01–16.5)
 Dst Dystonin 4.34 (0.01–15.4)
 Msh2 MutS homolog 2 (E. coli) 2.64 (0.01–9.35)
 Psmg2 Proteasome assembly chaperone 2 3.28 (0.01–10.3)
 Rbl1 Retinoblastoma-like protein 1 2.56 (0.01–7.06)
 Shc1 Src homology 2 transforming protein C1 2.21 (0.01–5.09)
 Trp63 Transformation related protein p63 2.37 (0.01–5.67)
Under-Expressed Genes
 Ccnb1 Cyclin B1 0.36 (0.01–1.04)
 Ccnb2 Cyclin B2 0.50 (0.19–0.82)
 Gpr132 G protein-coupled receptor 132 0.15 (0.01–0.76)
 Mcm3 Minichromosome maintenance complex-3 0.17 (0.01–0.70)
40%O2 Group—12 weeks (Control Group—21%O2 12 weeks)
Over-expressed genes
 Ccnb1 Cyclin B1 5.9 (0.01–10.75)
 Trp63 Transformation related protein p63 4.16 (0.01–11.59)
Under-expressed genes
 Abl1 Non-receptor tyrosine protein kinase 0.28 (0.01–0.67)
  1. Genes were considered over-expressed (Fold Change ≥ 2.0) or under-expressed (Fold Change ≤ 0.5) relative to the control group (21%O2). The fold change (FC) in gene expression in 21%O2 group = 1.0; values expressed as FC with 95% confidence intervals
  2. Housekeeping genes: Gapdh (Glyceraldehyde-3-phosphate dehydrogenase), Hprt (Hypoxanthine guanine phosphoribosyl transferase) & Actb (Actin, beta); N = six mice / group, each time-point
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