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Fig. 3 | Biological Research

Fig. 3

From: Daphnetin inhibits proliferation and inflammatory response in human HaCaT keratinocytes and ameliorates imiquimod-induced psoriasis-like skin lesion in mice

Fig. 3

The effect of daphnetin on the expression of inflammatory cytokines in M5-stimulated HaCaT keratinocytes. HaCaT keratinocytes were seeded in 12-well plates and treated with daphnetin (Dap, 20 μM) or not for 2 h, and subsequently stimulated with M5 cytokines (2.5 ng/ml) or not for 24 h. Cells were harvested and RNA was extracted for qRT-PCR analysis of IL-1β, IL-6, IL-8, TNF-α, IL-23A and MCP-1 mRNA levels. GAPDH served as an internal reference. *P < 0.05. **P < 0.01. DMSO (Ctl), DMSO + M5 (M5), daphnetin + M5 (M5 + Dap)

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