Fig. 2

The effect of M5 on the proliferation of HaCaT keratinocytes. a HaCaT keratinocytes were seeded in 96-well plates and stimulated with M5 (0, 1, 2.5 and 5 ng/ml) for 24–96 h. CCK-8 was used to measure cell viability. *P < 0.05, M5 (0 ng/ml) group vs M5 (2.5 ng/ml) group. b, c HaCaT keratinocytes were seeded in 12-well plates and stimulated with M5 (2.5 ng/ml) for 72 h. Cells were harvested and RNA was extracted for qRT-PCR analysis of KRT6 and KRT1 mRNA levels. GAPDH served as an internal reference. *P < 0.05. **P < 0.01