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Fig. 3 | Biological Research

Fig. 3

From: LncRNA OIP5-AS1 upregulates snail expression by sponging miR-34a to promote ovarian carcinoma cell invasion and migration

Fig. 3

OIP5-AS1 might sponge miR-34a to upregulate snail. To investigate the interaction between OIP5-AS1, miR-34a and snail, OIP5-AS1 expression vector, miR-34a mimic and snail expression vector were transfected into UWB1.289 cells. At 24 h post-transfection, the expression levels of OIP5-AS1, miR-34a and snail were measured by qRT-PCR (a). The interaction between miR-34a and OIP5-AS1 was predicted by IntaRNA (b). The interaction between OIP5-AS1 and miR-34a was analyzed by qRT-PCR (c). The effects of OIP5-AS1and miR-34a on the expression of snail were analyzed by qRT-PCR (d) and western blotting (e), respectively. Western blotting and qRT-PCR were repeated 3 times, and the mean values were expressed. NC1, empty pcDNA3 transfection; NC2, negative control miRNA transfection; *p < 0.05

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