Fig. 5

Glucose tolerance test (GTT) and glucose uptake assay. a Blood glucose was measured after a 7–8 h fast at baseline (0 min) and 10, 20, 30, 60 and 120 min after an i.p. injection of glucose (left), and AUC was calculated (right) (n = 9). b Plasma insulin concentration was measured during the GTT (left, n = 5–6). c The rates of basal and insulin-stimulated (150 nM) glucose uptake in SoL were determined with or without insulin 20 min after the addition of 2-DG in KHB containing 1 mM glucose. Arid5b^+/+ mice, n = 11–13; Arid5b^−/− mice, n = 8. d Glycogen content was quantitated in gastrocnemius muscle. Arid5b^+/+ mice, n = 10; Arid5b^−/− mice, n = 5. Results are expressed as the means ± SD. *, P < 0.05 vs Arid5b^+/+ mice; #, P < 0.05 vs insulin (-)