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Fig. 2 | Biological Research

Fig. 2

From: Increased glucose metabolism in Arid5b−/− skeletal muscle is associated with the down-regulation of TBC1 domain family member 1 (TBC1D1)

Fig. 2

Glucose oxidation was enhanced in Arid5b−/− skeletal muscles. a Oxygen consumption rates were measured and normalized to body weight (n = 5). *, P < 0.05. b Average distance traveled in two dark vs light periods of 24 h and total distance traveled in 48 h (n = 5) is shown. c Glucose oxidation rates and d fatty acid oxidation rates were measured in isolated skeletal muscles from non-fasted mice. Arid5b+/+ mice (n = 13–15) and Arid5b−/− mice (n = 5–6). *, P < 0.05. e Lactate levels and f ATP content was analyzed in GC muscles. Arid5b+/+ mice (n = 10) and Arid5b−/− mice (n = 5). *, P < 0.05; **, P < 0.01. g The phosphorylation levels of AMPKα1/2 at Thr172 and protein levels of total AMPKαα1/2 were normalized to loading control (HSP70), and the ratio of pAMPKα1/2 (Thr172) to total AMPKα1/2 was calculated. Values represent means ± SD. Arid5b+/+ mice, n = 7; Arid5b−/− mice, n = 5. #, HSP70 was detected in the same membrane as pAMPKα1/2 (Thr172)

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