Fig. 4
From: MiR-128 suppresses metastatic capacity by targeting metadherin in breast cancer cells
MTDH knockdown mimics miR-128-mediated phenotype and overexpression of MTDH restored miR-128-induced suppression of migration and invasion in MDA-MB-231 cells. a Immunoblotting analysis for expression of endogenous MTDH in MDA-MB-231 cells transfected with siNC and siMTDH-1/2. b, c Transwell migration and invasion assay of MDA-MB-231 cells transfected with siNC and siMTDH-1/2. Representative pictures of migrated or invaded cells with crystal violet staining are shown. Bars represent the relative migrated or invaded cells. d Western blot assay of MTDH in MDA-MB-231 cells cotransfected with NC or miR-128 mimics and pcDNA3.1-vector or pcDNA3.1-MTDH. e, f Transwell migration and invasion assay of MDA-MB-231 cells cotransfected with NC or miR-128 mimics and pcDNA3.1-vector or pcDNA3.1-MTDH. Representative pictures of migrated or invaded cells with crystal violet staining are shown. Bars represent the relative migrated or invaded cells. The symbol ** and *** represent P < 0.01 and P < 0.001, respectively, using a two-tailed Student’s t-test