Fig. 4

MATR3 suppresses EMT (epithelial-mesenchymal transition), migration, and invasion. a Real-time quantitative PCR was performed to measure relative mRNA levels of epithelial (Cytokeratin 18 and E-cadherin) and mesenchymal (N-cadherin, Vimentin and Fibronectin) markers in MDA-MB-231 (a introduced with empty vector and MATR3-overexpression vector) and BT549 cells (b introduced with non-silencing control and sh-MATR3). In vitro 3D migration and invasion of MDA-MB-231 (c introduced with empty vector and MATR3-overexpression vector) and BT549 (d introduced with non-silencing control and sh-MATR3) were examined using Boyden Chambers. Right panels in c and d show representative images of migration and invasion. Scale bars, 200 μm. n = 3 per group. Statistical significance in a–d was determined by an unpaired t-test. Error bars are s.e.m. * and **** indicate P < 0.05 and P < 0.0001, respectively