Skip to main content
Fig. 2 | Biological Research

Fig. 2

From: Tumor suppressive function of Matrin 3 in the basal-like breast cancer

Fig. 2

MATR3 promotes apoptotic cell death gauged by flow cytometry. a Cell cycle was analyzed in BT549 cells introduced with non-silencing control or short hairpin RNA against MATR3 (sh-MATR3) using propidium iodide by flow cytometry. b Sub-G1 cells were quantitated and compared between the BT549 cells introduced with non-silencing control and sh-MATR3. MDA-MB-231 (c, introduced with empty vector and MATR3-overexpression vector) and BT549 cells (f, introduced with non-silencing control and sh-MATR3) were cultured in the serum-free and growth factor-free medium for 24 h and subjected to flow cytometry following Annexin V and 7-AAD staining. (d and g) (Early, Annexin V +/7-AAD-, and late, Annexin V +/7-AAD +) apoptotic cells from c and f were quantitated and compared. (e and h) MDA-MB-231 (e introduced with empty vector and MATR3-overexpression vector) and BT549 cells (h introduced with non-silencing control and sh-MATR3) were treated with 0.5 μM Staurosporine for 24 h and subjected to flow cytometry following Annexin V and 7-AAD staining. Then (early and late) apoptotic cells were quantitated and compared. n = 3 per group. Statistical significance in b, d, e, g, and h was determined by an unpaired t-test. Error bars are s.e.m

Back to article page