Fig. 1

Comparison of MATR3 protein levels in a panel of basal-like/triple-negative human breast cancer cell lines. a Endogenous MATR3 protein levels were examined in a small panel of basal-like/triple-negative breast cancer cell lines by western blotting. The lower panel demonstrates the relative normalized MATR3 protein levels. b MATR3 was transiently overexpressed in MDA-MB-231 cells and the expression was determined by western blotting. The lower panel demonstrates the relative normalized MATR3 protein levels. c MATR3 was silenced by shRNAs and the expression was determined by western blotting. The lower panel demonstrates the relative normalized MATR3 protein levels. d In vitro cell proliferation was compared between control and MATR3-overexpressing MDA-MB-231 and between control and MATR3-depleted BT549 cells. n = 4 per group. e Anchorage-independent growth was examined to compare in vitro tumorigenesis by soft agar colony formation assay. n = 4 per group. Statistical significance in d and e was determined by an unpaired t-test. Error bars are s.e.m