Fig.4

NF-κB is required for VEGF transcription in gastric cancer cell with ablation of REC8. a Western blotting was used to detect phosphorylation of NF-κB. b A putative binding sequence of NF-κB p65 in VEGF promoter by UCSC analysis. c ChIP analysis of the binding of NF-κB to VEGF gene promoter in BGC823 cells depleted with REC8. Two-way ANOVA, **p < 0.01, the experiment was repeated three times. Error bars indicate mean ± S.D. d Western blotting and e–f tube formation were applied to measure VEGF in conditional medium from BGC823 cell infected with indicated lentivirus in present with or without NF-κB inhibitor BAY11-7082 treatment. g Schematic diagram for the mechanistic role of REC8 in tumor angiogenesis by regulation NF-κB-mediated VEGF in gastric cancer