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Fig. 4 | Biological Research

Fig. 4

From: LncRNA SNHG14 promotes OGD/R-induced neuron injury by inducing excessive mitophagy via miR-182-5p/BINP3 axis in HT22 mouse hippocampal neuronal cells

Fig. 4Fig. 4

SNHG14 overexpression promotes mitophagy of OGD/R-induced HT22 cells via miR-182-5p/BNIP3 axis. PcDNA3.1-SNHG14 or pcDNA3.1-NC and miR-182-5p mimic or mimic NC were co-transfected into HT22 cells. Then, the modified HT22 cells were subjected to OGD/R treatment. a QRT-PCR was performed to detect the expression of BNIP3 in the modified HT22 cells. b Flow cytometry was performed to explore the apoptosis of the modified HT22 cells. c The expression of BNIP3, caspase-3, cleaved-caspase-3, caspase-9 and cleaved-caspase-9 in the modified HT22 cells was examined by WB. PcDNA3.1-SNHG14 or pcDNA3.1-NC and si-BNIP3 or si-Ctrl were co-transfected into HT22 cells. Then, the modified HT22 cells were subjected to OGD/R treatment. d QRT-PCR was performed to detect the expression of BNIP3 in the modified HT22 cells. e Flow cytometry was performed to explore the apoptosis of the modified HT22 cells. f The expression of BNIP3, caspase-3, cleaved-caspase-3, caspase-9 and cleaved-caspase-9 in the modified HT22 cells was examined by WB. (*P < 0.05, **P < 0.01, versus mimic NC + Vector or Vector + si-Ctrl; #P < 0.05, ##P < 0.01, versus Vector + miR-182-5p mimic Vector + si-BNIP3)

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