Fig. 3

SNHG14 regulates BNIP3 expression by sponging miR-182-5p. HT22 cell model was established by OGD/R treatment. Normal HT22 cells served as control. a QRT-PCR was performed to assess the expression of miR-182-5p in the HT22 cells. b, c QRT-PCR and WB were performed to estimate the expression of BNIP3 in the HT22 cells. HT22 cells were transfected with pcDNA3.1-SNHG14, pcDNA3.1-NC, si-SNHG14 or si-Ctrl. d, e QRT-PCR was performed to detect the expression of miR-182-5p and BNIP3 in the modified HT22 cells. f The Wt (Mut) SNHG14 vector or Wt (Mut) BNIP3 vector and miR-182-5p mimic or mimic NC were co-transfected into 293T cells. The interaction among SNHG1, miR-182-5p and BNIP3 was measured by luciferase reporter assay. HT22 cells were transfected with miR-182-5p mimic, mimic NC, miR-182-5p inhibitor or inhibitor NC. g QRT-PCR was performed to assess the expression of SNHG14 in the modified HT22 cells. h, i The gene and protein expression of BNIP3 in the modified HT22 cells was explored by qRT-PCR or WB. (*P < 0.05, **P < 0.01, versus Vector or mimic NC; ^#P < 0.05, ^##P < 0.01, versus si-Ctrl or inhibitor NC)