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Fig. 4 | Biological Research

Fig. 4

From: Curcumin prevents neuronal loss and structural changes in the superior cervical (sympathetic) ganglion induced by chronic sleep deprivation, in the rat model

Fig. 4

TUNEL assay of the superior cervical ganglion. AD Diaminobenzidine detection by light microscope. a–d TUNEL assay with fluorescence microscope by excitation wavelength in the range of 450–500 nm; Arrow indicated apoptotic cells. A&a A normal appearance and low apoptotic cell can be seen in the cage-control. B&b In the curcumin-treated animals, the apoptotic cell was not observed. C&c An apparent increase in the number of apoptotic cells was observed within chronic sleep deprivation (CSD) animals compared with the control groups. D&d The number of apoptotic cells in the CSD animals receiving curcumin appeared to be similar to the control group. E The negative control, the TdT enzyme was removed from the TdT reaction buffer that did not display TUNEL-positive cells. F Positive control, the rat thymus with hydrocortisone-induced apoptosis thymocytes used as the positive control. A large number of apoptotic cells can be seen in this tissue. G The mean ± SEM of apoptotic cell percentage in the different groups

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