Fig. 3

Edaravone attenuated IR-induced kidney mitochondrial injury. a Ultrastructural of mitochondria was analyzed by transmission electron microscopy (×20,000). b Flameng semi-quantitative assessment of the mitochondria ultrastructural based on mitochondrial damage. c JC-1 dye was used to detect the mitochondrial membrane potential (ΔΨm) in each group by fluorescence microplate reader. The up bar graph was the ratio of red/green fluorescence indicated the ΔΨm level and the below bar graph was the ΔΨm standardized by sham. d, e The mitochondrial function was evaluated by CS enzyme activity and ATP, the up bar graph was the level of CS enzyme activity and ATP in the each group, and the below bar graph was the level of CS enzyme activity and ATP normalized by the sham data in each group. Data are represented as mean ± SEM (n = 10 per group) *P < 0.05, **P < 0.01, ***P < 0.001 vs. sham; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. IR