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Fig. 5 | Biological Research

Fig. 5

From: Silencing of circular RNA ANRIL attenuates oxygen–glucose deprivation and reoxygenation-induced injury in human brain microvascular endothelial cells by sponging miR-622

Fig. 5

Silencing of circ_ANRIL attenuates OGD/R-induced cell damage and inflammatory responses by modulating miR-622. a Downregulation of miR-622 was induced by transfection of miR-622 inhibitor and then its expression was detected by qRT-PCR. si-circ_ANRIL (or si-NC) and miR-622 inhibitor (or NC-inhibitor) were transfected into HBMECs, either alone or in combination. The transfected HBMECs were then exposed to OGD/R. Conventionally cultured HBMECs were used as controls. b The expression of miR-622 was detected by qRT-PCR. c–f Transfection with si-circ_ANRIL improved cell viability, reduced cytotoxicity and apoptosis, accompanied by the changes in protein expression of Bcl-2 and Bax. g–j Silencing of circ_ANRIL inhibits inflammatory factor release in OGD/R-treated HBMECs. Whereas, co-transfection with miR-622 inhibitor counteracted these effects of si-circ_ANRIL on OGD/R-induced HBMECs. *p < 0.05, **p < 0.01, ***p < 0.001. n = 3

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