Fig. 5

PLD2 inhibitor promotes SAHA-induced suppression of invasion and migration. a MDA-MB-231 and MDA-MB435 cells were seeded in Matrigel-coated invasion chambers and then treated with SAHA and/or PLD2 inhibitor for 24 h. The extent of invasion was expressed as an average number of cells per microscopic field. After 24 h, the conditioned medium was collected, applied to HUVECs, and migration b and tube formation c were determined. The extent of migration is expressed as the average number of cells per microscopic field. Results are representative of at least four independent experiments and shown as the mean ± SEM. **p < 0.001