Fig. 4

PLD2 inhibition sensitizes SAHA-induced apoptotic cell death. a MDA-MB-231 cells were transfected with siRNA of PLD2 and then treated with vehicle or SAHA (5 μM) for 36 h. Apoptosis was examined via flow cytometry and presented as the percentage of cells labeled with annexin V-PE. b MDA-MB-231 cells were stained with propidium iodide (1 μg/mL) and the population of subG1 apoptotic cells was determined using FACScan flow cytometry. c MDA-MB231 cells were treated with T247 (3 μM), TMP195 (3 μM), and PLD2 inhibitor (10 μM) for 36 h, and caspase-3 activity was examined. d MDA-MB231 were treated with SAHA (5 μM), rapamycin (50 nM), temozolomide (50 μM), and PLD2 inhibitor (10 μM) for 36 h, and caspase-3 activity was examined. e The cells were treated with SAHA, PLD2 inhibitor, or PA (50 μM) for 36 h. Apoptosis using annexin V staining was examined. f MDA-MB-231 cells were treated with the indicated drugs for 36 h, and caspase-3 activity and g expression of the indicated proteins were examined. The intensity of the indicated bands was normalized to the intensity of their respective α-tubulin bands and quantified against each other. Results are representative of at least four independent experiments and shown as the mean ± SEM. **p < 0.001