|
Loci
|
Forward primer sequences(5′ to 3′)
|
Reverse primer sequences(5′ to 3′)
|
Predicted size
|
Motif
|
Repeats
|
A
|
Ho
|
He
|
H’
|
|---|
|
Loci01
|
CGATAGCGAGAAGAGATACGGG
|
AGAGAAAAATCAGGCCAGTCCA
|
248
|
CA
|
7
|
16
|
0.3564
|
0.8693
|
2.2622
|
|
Loci02
|
CGGGGTCTTGATTTTGGAGAGA
|
CTGTAGACGTGCTCTTCCGATT
|
269
|
AG
|
10
|
8
|
0.3084
|
0.4916
|
1.0601
|
|
Loci03
|
GTTTTCTCCAATGCTCCACACC
|
CTCTATAGTCCTCGTGTCGCAC
|
253
|
AG
|
13
|
24
|
0.2909
|
0.9344
|
2.9145
|
|
Loci04
|
CGTTTCAAATAGGTGGGAGGGA
|
TGCTGTCCCAAAGCTTCACTAA
|
256
|
CT
|
7
|
21
|
0.7532
|
0.913
|
2.6479
|
|
Loci05
|
AGAGGAAGTGGAGGAAGAAGGA
|
TGCCCTCATTTATCTCTCTCGC
|
168
|
AG
|
13
|
12
|
0.1101
|
0.1919
|
0.5557
|
|
Loci06
|
TCGAGTTGGCGAGTAATTGTCA
|
TCTTTGTCGCTTTCTCTCCCTC
|
250
|
AG
|
14
|
21
|
0.573
|
0.9156
|
2.6439
|
|
Loci07
|
ATGTCCAGTCGTAGAAGGGAGA
|
ATCTTACAAATTCCCCCTGGGC
|
280
|
TC
|
13
|
27
|
0.7733
|
0.9023
|
2.7063
|
|
Loci08
|
CCAAGACGAGAACGATCGATCT
|
AAGTGAGAAAATGCACGTGGTG
|
157
|
TC
|
7
|
19
|
0.7113
|
0.8737
|
2.4331
|
|
Loci09
|
AGGGGATTACTGACGTCGAGTA
|
GAGTATCATAGGCCCATTACCCT
|
260
|
AG
|
13
|
28
|
0.3486
|
0.9198
|
2.8313
|
|
Loci10
|
GGATTTAGTTCGGGGAAGACGT
|
TAGGGCCGTTTGCAACATTTTT
|
236
|
CT
|
7
|
22
|
0.1972
|
0.9059
|
2.606
|
|
Loci11
|
CATGCCAGGCCTGTTAAAAGTC
|
GCTAGCTCTGACAGGCTTCTAG
|
179
|
GT
|
7
|
25
|
0.7944
|
0.9034
|
2.5901
|
|
Loci12
|
GGCACAGATCAAAAATCGCACT
|
CTTCCATGCCTCTCCGCCATTA
|
140
|
GA
|
18
|
24
|
0.7156
|
0.7804
|
1.9415
|
|
Loci13
|
CAACCTTCTCTGTCACCTCCG
|
ATAAGTAGTGGAGAGCATGCGG
|
145
|
TC
|
14
|
26
|
0.486
|
0.9221
|
2.8333
|
|
Mean
| | | | | |
21
|
0.4937
|
0.8095
|
2.3097
|
- A number of alleles, Ho observed heterozygosity, He expected heterozygosity, H’ Shannon‐Wiener diversity
