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Fig. 1 | Biological Research

Fig. 1

From: Engraftment of human induced pluripotent stem cell-derived myogenic progenitors restores dystrophin in mice with duchenne muscular dystrophy

Fig. 1

Generation and characterization of EGFP hiPSCs. a Phase contrast and fluorescence micrographs showed morphology and GFP expression in hiPSCs transduced with the EGFP expression vector. b EGFP hiPSCs retained normal karyotype. c Immunofluorescence analyses of the pluripotent markers, OCT4, SOX2, TRA-1-60, and TRA-1-81, in EGFP hiPSCs. d EGFP hiPSCs differentiated into three germ layers via EB formation, as indicated by the expression of AFP, αSMA, and Nestin in immunofluorescence staining. e Hematoxylin and eosin (H&E) staining of teratoma formed from EGFP hiPSCs showed endoderm-derived glandular epithelium, mesoderm-derived smooth muscle, and ectoderm-derived neural tube. Scale bars = 100 μm

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