Fig. 5

LINC00483 sponges miR-490-3p to regulate MAPK1 expression in gastric cancer cells. a The complementary sequences between miR-490-3p and LINC00483 or MAPK1 were predicted by miRcode or TargetScan. b, c Luciferase activity was measured in MKN-45 and MGC-803 cells co-transfected with WT-LINC00483 or MUT-LINC00483 and miR-490-3p or miR-NC. d The effect of LINC00483 on miR-490-3p level in MKN-45 and MGC-803 cells was investigated. e, f Luciferase reporter assay was performed in MKN-45 and MGC-803 cells co-transfected with MAPK1 3′UTR-WT or MAPK1 3′UTR-MUT and miR-490-3p or miR-NC. g, h The regulatory effect of miR-490-3p and LINC00483 on MAPK1 protein level was assessed in MKN-45 and MGC-803 cells. i, j The expression of miR-490-3p was detected in gastric cancer tissues (n = 30) and cells. k, l The association of levels of miR-490-3p and LINC00483 or MAPK1 in gastric cancer tissues was analyzed. Cell viability (m), apoptosis (n), migration (o) and invasion (p) were measured in MKN-45 and MGC-803 cells transfected with miR-NC, miR-490-3p, miR-490-3p and pcDNA or LINC00483 via MTT, flow cytometry and transwell assay. WT: wild-type; MUT: mutant; miR-490-3p: miR-490-3p mimic; miR-NC: miRNA negative control; LINC00483: LINC00483 overexpression; pcDNA: empty vector for overexpressing genes. *P < 0.05 compared with matched group