Fig. 4

MAPK1 overexpression reverses the effect of LINC00483 knockdown on cell proliferation, apoptosis, migration and invasion in gastric cancer cells. a–d The mRNA and protein levels of MAPK1 were determined in MKN-45 and MGC-803 cells transfected with sh-LINC00483-1, sh-NC, LINC00483 or pcDNA via qRT-PCR and western blot. Cell viability (e, f), apoptosis (g, h), migration (i, j), invasion (k, l) and related protein levels (M and N) were examined in MKN-45 and MGC-803 cells transfected with sh-NC, sh-LINC00483-1, sh-LINC00483-1 and pcDNA or MAPK1 via MTT, flow cytometry, transwell assay and western blot. sh-LINC00483-1: LINC00483 knockdown using shRNA; sh-NC: shRNA negative control; LINC00483: LINC00483 overexpression; MAPK1: LINC00483; pcDNA: empty vector for overexpressing genes. *P < 0.05 compared with matched group