Fig. 5

Silenced miR-19a-3p exerted protective roles against OGD/R-induced injury by up-regulating IGFBP3. SH-SY5Y cells were transfected with empty vector, IGFBP3, inhibitor or inhibitor plus si-IGFBP3, respectively, followed by OGD/R exposure. a The protein level of IGFBP3 expression was detected by western blot analysis. b Cell viability, c–e pro-inflammatory cytokines and f–g apoptosis were evaluated in SH-SY5Y cells after the above treatments using CCK-8, ELISA and flow cytometry assays, respectively. The experiments were performed in triplicate and each value represented mean ± SD. **p < 0.01, ***p < 0.001, compared with Vector; ###p < 0.01, compared with inhibitor