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Fig. 5 | Biological Research

Fig. 5

From: Inhibition of miR-19a-3p decreases cerebral ischemia/reperfusion injury by targeting IGFBP3 in vivo and in vitro

Fig. 5

Silenced miR-19a-3p exerted protective roles against OGD/R-induced injury by up-regulating IGFBP3. SH-SY5Y cells were transfected with empty vector, IGFBP3, inhibitor or inhibitor plus si-IGFBP3, respectively, followed by OGD/R exposure. a The protein level of IGFBP3 expression was detected by western blot analysis. b Cell viability, ce pro-inflammatory cytokines and fg apoptosis were evaluated in SH-SY5Y cells after the above treatments using CCK-8, ELISA and flow cytometry assays, respectively. The experiments were performed in triplicate and each value represented mean ± SD. **p < 0.01, ***p < 0.001, compared with Vector; ###p < 0.01, compared with inhibitor

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