Fig. 4

IGFBP3 3′-untranslated region (UTR) was directly targeted by miR-19a-3p. a Schema of the WT and mutated IGFBP3 3′-UTR indicating the interaction sites between miR-19a-3p and the 3′-UTR of IGFBP3. b Dual luciferase assay in SH-SY5Y cells co-transfected with the miR-19a-3p inhibitor and reporter vectors containing either the wild-type or mutated 3’-UTR of IGFBP3. c RT-qPCR and d Western blot analysis were used to determine the expression of IGFBP3 in SH-SY5Y cells transfected with miR-19a-3p inhibitor or miR-NC, and then was exposed to OGD/R conditions. **p < 0.01, compared with miR-NC; ###p < 0.001, compared with Normoxia; The expression levels of IGFBP3 mRNA (e) and protein (f) were determined in Sham rat brains and I/R rat brains treated with miR-19a-3p inhibitor. The experiments were performed in triplicate and each value represented mean ± SD. ***p < 0.01, compared with Sham; ##p < 0.01, compared with MCAO