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Fig. 3 | Biological Research

Fig. 3

From: Inhibition of miR-19a-3p decreases cerebral ischemia/reperfusion injury by targeting IGFBP3 in vivo and in vitro

Fig. 3

Down-regulation of miR-19a-3p protected SH-SY5Y cells against OGD/R-induced injury. SH-SY5Y cells were transfected with miR-19a-3p inhibitor or miR-NC, followed by OGD/R exposure. The expression of miR-19a-3p was determined in (a) Normoxia and OGD/R group, as well as (b) OGD/R + miR-NC and OGD/R + inhibitor group. c CCK-8 assay was utilized to analyze cell viability. The levels of TNF-α (d), IL-1β (e), and IL-6 (f) were measured by ELISA assay. g Cell apoptosis was determined with Annexin V/PI double staining followed by flow cytometry assay. The experiments were performed in triplicate and each value represented mean ± SD. ***p < 0.001, compared with Normoxia; ##p < 0.01, ###p < 0.001, compared with OGD/R + miR-NC

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