Fig. 2

The effect of miR-19a-3p on pro-inflammatory cytokines and apoptosis in I/R rat brain. The levels of TNF-α a, IL-1β b, and IL-6 c in the Sham, MCAO, and MCAO + inhibitor groups were measured by ELISA (n = 6 per group). d The apoptosis of cortical neurons was evaluated by TUNEL staining. e–f Measurement of Bcl-2 and Bax protein levels in Sham, MCAO and MCAO + inhibitor groups using western blotting. The experiments were performed in triplicate and each value represented mean ± SD. ***p < 0.001, compared with Sham; ##p < 0.01, ###p < 0.001, compared with MCAO