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Fig. 1 | Biological Research

Fig. 1

From: Mast cells-derived MiR-223 destroys intestinal barrier function by inhibition of CLDN8 expression in intestinal epithelial cells

Fig. 1

MCs-secreted exosomes were internalized and destroyed intestinal barrier function. a Western blotting analysis of indicated proteins in HMCs-1, NCM460 and exosomes. b Indicated IECs were incubated with PKH67-labelled exosomes (green) for 24 h before fluorescent and phase contrast images were captured. c The permeability of treated CaCO2 monolayers grown on 0.4 mm filters was measured by the appearance of rhodamine-dextran, which was added to the top well at the beginning of the experiment, in the bottom well during a 1 h time course. The absorbance at the 1 h time point was compared with the PBS (control) condition. ***p < 0.001. d IF analysis of CLDN 8, OCLN and ZO-1 in CaCO2 cells treated with or without exosomes. e Western blotting analysis of the changes of tight junction protein in CaCO2 and NCM460 cells treated as indicated

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