Fig. 7

SYVN1 knockdown impaired the effect on cell proliferation, apoptosis and autophagy caused by anti-miR-34a-5p. a, b The mRNA a and protein b expression of SYVN1 was detected in OA cells (IL-1β) transfected with anti-NC, anti-miR-34a-5p, anti-miR-34a-5p + scramble and anti-miR-34a-5p + siSYVN1 by qRT-PCR and western blot. c Cell proliferation was measured in OA cells (IL-1β) transfected with anti-NC, anti-miR-34a-5p, anti-miR-34a-5p + scramble and anti-miR-34a-5p + siSYVN1 after transfection 24 h, 48 h, 72 h by MTT assay. d Cell apoptosis was detected in OA cells (IL-1β) transfected with anti-NC, anti-miR-34a-5p, anti-miR-34a-5p + scramble and anti-miR-34a-5p + siSYVN1 by flow cytometry. e The protein expression of PCNA and cleaved-caspase 3 were measured in OA cells (IL-1β) transfected with anti-NC, anti-miR-34a-5p, anti-miR-34a-5p + scramble and anti-miR-34a-5p + siSYVN1 by western blot. f The protein expression of beclin1 and LC3 were measured in OA cells (IL-1β) transfected with anti-NC, anti-miR-34a-5p, anti-miR-34a-5p + scramble and anti-miR-34a-5p + siSYVN1 by western blot. *P < 0.05