Fig. 4

miR-34-5p was a target miRNA of SNHG7. a The predicted binding sites of miR-34a-5p to SNHG7 sequence using starBase 2.0. b The luciferase activity of 293T cells co-transfected with miR-34a-5p and luciferase reporters containing SNHG7-wt or SNHG7-mut was detected using luciferase reporter assay. c The luciferase activity of 293T cells co-transfected with anti-miR-34a-5p and luciferase reporters containing SNHG7-wt or SNHG7-mut was detected using luciferase reporter assay. d The expression of SNHG7 was detected in the samples bound to the Ago2 antibody by RIP assay. e The expression of SNHG7 was detected using qRT-PCR in samples pulled down by biotinylated miR-34a-5p or negative control (NC). f The expression of miR-34a-5p was detected in OA cells (IL-1β) transfected with control, vector, SNHG7, scramble and siSNHG7 by qRT-PCR. g Pearson’s correlation analysis revealed the significant negative correlation between SNHG7 and miR-34a-5p expression. *P < 0.05