Fig. 3

Down-regulation of miR-34a-5p promoted cell proliferation as well as inhibited cell apoptosis and autophagy. a The expression of miR-34a-5p in OA tissues and normal tissues was detected by qRT-PCR. b The expression of miR-34a-5p in OA cells stimulated 10 ng/ml IL-1β and OA cells. c The expression of miR-34a-5p was detected in OA cells transfected with control, anti-NC and anti-miR-34a-5p by qRT-PCR. d Cell proliferation was measured in OA cells (IL-1β) transfected with control, anti-NC and anti-miR-34a-5p after transfection 24 h, 48 h, 72 h by MTT assay. e Cell apoptosis was detected in OA cells (IL-1β) transfected with control, anti-NC and anti-miR-34a-5p by flow cytometry. f The protein expression of PCNA and cleaved-caspase 3 were measured in OA cells (IL-1β) transfected with control, anti-NC and anti-miR-34a-5p by western blot. g The protein expression of beclin1 and LC3 were measured in OA cells (IL-1β) transfected with control, anti-NC and anti-miR-34a-5p by western blot. *P < 0.05